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Ukraine President Petro Poroshenko says the country has barred Russian men between 16 and 60 from travelling to the country.
The move comes as the long-simmering conflict between the two nations escalated in the Black Sea on Sunday.
Russian border guards opened fire on and captured three Ukrainian vessels and their 24-member crew, reports AP.
Mr Poroshenko tweeted Friday that the restrictions on Russian travellers have been taken in order to prevent the Russians from forming "private armies" fighting on Ukrainian soil.
A Russian government-appointed ombudswoman says the three commanders of the Ukrainian vessels captured near the Russia-annexed Crimean Peninsula last weekend are being transferred to Moscow.
The conflict between Russia and Ukraine bubbled over Sunday when Russian border guards opened fired on three Ukrainian vessels in the Black Sea. The Russians then captured the 24-member crew.
Russia says the Ukrainians had violated its border while Ukraine says its ships were acting in line with international maritime rules.
The Tass news agency on Friday quoted Russian government-appointed ombudswoman Lyudmila Lubina as saying that the vessels' commanders are being transferred to Moscow for interrogation.
The other 21 remain in custody in Crimea.
A Crimea court earlier this week ruled to keep the Ukrainian seamen behind bars for two months pending the investigation.